Publications: Department of Immunology

2024

Nonspecific lipid-transfer proteins trigger TLR2 and NOD2 signaling and undergo ligand-dependent endocytosis in epithelial cells.

Cavallari N, Johnson A, Nagl C, Seiser S, Rechberger GN, Züllig T, Kufer TA, Elbe-Bürger A, Geiselhart S, Hoffmann-Sommergruber K.

J Allergy Clin Immunol. 2024 Jul 29:S0091-6749(24)00744-9. doi: 10.1016/j.jaci.2024.07.015.

DnaRNA-LL37 composite DAMPs define sterile NETs as self-propagating drivers of inflammation.

Bork F, Greve CL, Youn C, Chen S, N C Leal V, Wang Y, Fischer B, Nasri M, Focken J, Scheurer J, Engels P, Dubbelaar M, Hipp K, Zalat B, Szolek A, Wu MJ, Schittek B, Bugl S, Kufer TA, Löffler MW, Chamaillard M, Skokowa J, Kramer D, Archer NK, Weber ANR.

EMBO Rep. 2024 May 23. doi: 10.1038/s44319-024-00150-5

2023

NOD1 cooperates with HAX-1 to promote cell migration in a RIPK2- and NF-ĸB-independent manner

Lucy Hezinger, Sarah Bauer, Kornelia Ellwanger, Alban Piotrowsky, Felix Biber, Sascha Venturelli, Thomas A. Kufer
The FEBS Journal, doi: 10.1111/febs.16912

NOD1 mediates interleukin-18 processing in epithelial cells responding to Helicobacter pylori infection in mice.

Tran LS, Ying L, D'Costa K, Wray-McCann G, Kerr G, Le L, Allison CC, Ferrand J, Chaudhry H, Emery J, De Paoli A, Colon N, Creed S, Kaparakis-Liaskos M, Como J, Dowling JK, Johanesen PA, Kufer TA, Pedersen JS, Mansell A, Philpott DJ, Elgass KD, Abud HE, Nachbur U, Croker BA, Masters SL, Ferrero RL.Nat Commun. 2023 Jun 26;14(1):3804. doi: 10.1038/s41467-023-39487-1.

Characterization of the Inflammatory Response Evoked by Bacterial Membrane Vesicles in Intestinal Cells Reveals an RIPK2-Dependent Activation by Enterotoxigenic Escherichia coli Vesicles.

Thapa HB, Kohl P, Zingl FG, Fleischhacker D, Wolinski H, Kufer TA, Schild S.
Microbiol Spectr. 2023 Jun 12:e0111523.
doi: 10.1128/spectrum.01115-23.

NOD-like Receptors - Emerging Links to Obesity and Associated Morbidities.

Sarah Bauer, Lucy Hezinger, Fjolla Pexhepi, Sheela Ramanthan, Thomas A. Kufer
Int J Mol Sci. 2023 24(10):8595

NLRC5-CIITA Fusion Protein as an Effective Inducer of MHC-I Expression and Antitumor Immunity.

Santharam MA, Shukla A, Levesque D, Kufer TA, Boisvert FM, Ramanathan S, Ilangumaran S.
Int J Mol Sci. 2023 Apr 13;24(8):7206.

Mitochondrial damage activates the NLRP10 inflammasome.

Próchnicki T, Vasconcelos MB, Robinson KS, Mangan MSJ, De Graaf D, Shkarina K, Lovotti M, Standke L, Kaiser R, Stahl R, Duthie FG, Rothe M, Antonova K, Jenster LM, Lau ZH, Rösing S, Mirza N, Gottschild C, Wachten D, Günther C, Kufer TA, Schmidt FI, Zhong FL, Latz E.
Nat Immunol. 2023 Mar 20. doi: 10.1038/s41590-023-01451-y.

NLRC5 affects diet-induced adiposity in female mice and co-regulates peroxisome proliferator-activated receptor PPARγ target genes,
Bauer S, Aeissen V, Bubeck AM, Kienes I, Ellwanger K, Scheurenbrand M, Rexhepi F, Ramanathan S, Rosenstiel P, Fricke WF, Kufer TA,
iScience, 2023, 106313, doi: 10.1016/j.isci.2023.106313.iSicence

Editorial: Updates on RIG-I-like receptor-mediated innate immune responses.

Kishore U, Kufer TA. Front Immunol. 2023 Feb 8;14:1153410. doi: 10.3389/fimmu.2023.1153410.

2022

Prumnopitys Andina Fruit Extract Activates Liver X Receptors After In Vitro Digestion. Jiménez-Aspee F, Pospiech J, Bauer S, Sus N, Kufer TA, Frank J. Mol Nutr Food Res. 2022 Oct 20:e2200377.
doi: 10.1002/mnfr.202200377.

Selective autophagy of RIPosomes maintains innate immune homeostasis during bacterial infection.

Mehto S, Jena KK, Yadav R, Priyadarsini S, Samal P, Krishna S, Dhar K, Jain A, Chauhan NR, Murmu KC, Bal R, Sahu R, Jaiswal P, Sahoo BS, Patnaik S, Kufer TA, Rusten TE, Chauhan S, Prasad P, Chauhan S.EMBO J. 2022 Oct 11:e111289. doi: 10.15252/embj.2022111289.

Analysis of the Localization of NLRs upon Shigella flexneri Infection Exemplified by NOD1.

Arnold C, Ellwanger K, Kufer TA.
Methods Mol Biol. 2022;2421:37-56. doi: 10.1007/978-1-0716-1944-5_3

A Brief Introduction to Effector-Triggered Immunity.

Kufer TA, Kaparakis-Liaskos M.
Methods Mol Biol. 2022;2523:1-8. doi: 10.1007/978-1-0716-2449-4_1.PMID: 35759187

Assaying RIPK2 Activation by Complex Formation.

Steinle H, Ellwanger K, Kufer TA.
Methods Mol Biol. 2022;2523:133-150. doi: 10.1007/978-1-0716-2449-4_9. PMID: 35759195

Bacterial subversion of NLR-mediated immune responses.

Kienes I, Johnston EL, Bitto NJ, Kaparakis-Liaskos M, Kufer TA. Front Immunol. 2022 Jul 28;13:930882. doi: 10.3389/fimmu.

2021

NLRC5 Deficiency Deregulates Hepatic Inflammatory Response but Does Not Aggravate Carbon Tetrachloride-Induced Liver Fibrosis.

Quenum AJI, Shukla A, Rexhepi F, Cloutier M, Ghosh A, Kufer TA, Ramanathan S, Ilangumaran S.

Front Immunol. 2021 Oct 12;12:749646. doi: 10.3389/fimmu.2021.749646.

14-3-3 and Erlin proteins differentially interact with RIPK2 complexes.

Steinle H, Ellwanger K, Mirza N, Brise S, Kienes I, Pfannstiel J, Kufer TA.

J Cell Sci. 2021 Jun 4:jcs.258137. doi: 10.1242/jcs.258137.

DDX3X Links NLRP11 to the Regulation of Type I Interferon Responses and NLRP3 Inflammasome Activation.
Kienes I, Bauer S, Gottschild C, Mirza N, Pfannstiel J, Schröder M, Kufer TA

Front. Immunol. 2021 May 12:653883. doi: 10.3389/fimmu.2021.653883

Cellular stress promotes NOD1/2-dependent inflammation via the endogenous metabolite sphingosine-1-phosphate.

Pei G, Zyla J, He L, Moura-Alves P, Steinle H, Saikali P, Lozza L, Nieuwenhuizen N, Weiner J, Mollenkopf HJ, Ellwanger K, Arnold C, Duan M, Dagil Y, Pashenkov M, Boneca IG, Kufer TA, Dorhoi A, Kaufmann SH.

EMBO J. 2021 May 4:e106272.doi: 10.15252/embj.2020106272.

Role of NLs in the Regulation of Type I Interferon Signaling, Host Defense and Tolerance to Inflammation.

Kienes I, Weidl T, Mirza N, Chamaillard M, Kufer TA

Int J Mol Sci. 2021 Jan 28;22(3):1301. doi: 10.3390/ijms22031301.

Detection of Bacterial Membrane Vesicles by NOD-Like Receptors.

Johnston EL, Heras B, Kufer TA, Kaparakis-Liaskos M.

Int J Mol Sci. 2021 Jan 20;22(3):E1005. doi: 10.3390/ijms22031005.

2020

Targeting the innate immunoreceptor RIG-I overcomes melanoma-intrinsic resistance to T cell immunotherapy.

Such L, Zhao F, Liu D, Thier B, Le-Trilling VTK, Sucker A, Coch C, Pieper N, Howe S, Bhat H, Kalkavan H, Ritter C, Bringhaus R, Ugurel S, Köster J, Seifert U, Dittmer U, Schuler M, Lang KS, Kufer TA, Hartmann, G, Becker JC, Horn S, Ferrone S, Liu D, Van Allen EM, Schadendorf D, Griewank K, Trilling M, Paschen A.

J Clin Invest. 2020 May 19:131572. doi: 10.1172/JCI131572

Innate Immune Molecule NLRC5 Protects Mice From Heliobacter-induced Formation of Gastric Lymphoid Tissue.

Chonwerawong M, Ferrand J, Chaudhry HM, Higgins C, Tran LS, Lim SS, Walker MM, Bhathal PS, Dev A, Moore GT, Sievert W, Jenkins BJ, D'Elios MM, Philpott DJ, Kufer TA, Ferrero RL

Gastroenterology. 2020 Mar 10. pii: S0016-5085(20)303329. doi:10.1053/j.gastro.2020.03.009

Cytosolic Gram-negative bacteria prevent apoptosis by inhibition of effector caspases through lipopolysaccaride.

Günther SD, Fritsch M, Seeger JM, Schiffmann LM, Snipas SJ, Coutelle M, Kufer TA, Higgins PG, Hornung V, Bernardini ML, Höning S, Krönke M, Salvesen GS, Kashkar H.

Nat Microbiol. 2019 Dec 23. doi: 10.1038/s41564-019-0620-5.

Bacterial Membran Vesicles: Biogenesis, Functions and Applications

edited by Maria Kaparakis-Liaskos and Thomas A. Kufer

Springer International Publishing AG, 2020

ProQuest Ebook Central, ebookcentral.proquest.com/lib/ubhohenheim/detail.action.

Immunodetection and Pathogenesis Mediated by Bacterial Membrane Vesicles

Ella L. Johnson, Thomas A. Kufer and Maria Kaparakis-Liaskos in

Bacterial Membrane Vesicles: Biogenesis, Functions and Applications

2019

Guardians of the Cell: Effecotr-Triggered Immunity Steers Mammalian Immune Defense.

Kufer TA, Creagh EM, Bryant CE

Trends Immunol. 2019 Sep 6. pii: S1471-4906(19)30168-1.

doi: 10.1016/j.9t.2019.08.001

XIAP controls RIPK2 signaling by preventing its depostion in speck-like structures.

Ellwanger K, Briese S, Arnold C, Kienes I, Heim V, Nachbur U, Kufer TA

Life Sci Alliance. 2019 Jul 26;2(4). pii: e201900346. doi: 10.26508/lsa.201900346:

Angeborene Immunität und Adipositas - Die Rolle der Nod-like Rezeptoren (NLR)

Mirza et al. Aktuel Ernahrungsmed 2019; 44: 248-260 (peer-reviewed)

Programmed necotic cell death of macrophages: Focus on pyroptosis, necroptosis, and pathanatos

Nirmal Robinson, Raja Ganesan, Csaba Hegedus, Katalin Kovács, Thomas A. Kufer, Lászlo Virág

Redox Biology, Elsevier, Juni 2019

doi.org/10.1016/j.redox.2019.101239

2018

Proteasomal degradation of NOD2 by NLRP12 in monocytes promotes bacterial tolerance and colonization by enteropathogens.

Normand S, Waldschmitt N, Neerincx A, Martinez-Torres RJ, Chauvin C, Couturier-Maillard A, Boulard O, Cobret L, Awad F, Huot L, Ribeiro-Ribeiro A, Lautz K, Ruez R, Delacre M, Bondu C, Guilliams M, Scott C, Segal A, Amselem S, Hot D, Karabina S, Bohn E, Ryffel B, Poulin LF, Kufer TA, Chamaillard M.

Nat Commun. 2018 Dec 17;9(1):5338. doi: 10.1038/s41467-018-07750-5.

NLRP10 Affects the Stability of Abin-1 to Control Inflammatory Responses

Nora Mirza, Anna S Sowa, Katja Lautz, Thomas A. Kufer

J Immunol December 3, 2018, ji1800334; DOI: doi.org/10.4049/jimmunol.1800334 PMID: 30510071

Subcellular antigen localization in commensal E. coli is critical for T cell activation and induction of specific tolerance.

Bennek E, Mandic AD, Verdier J, Roubrocks S, Pabst O, Van Best N, Benz I, Kufer T, Trautwein C, Sellge G.

Mucosal Immunol. 2018 Oct 16., doi: 10.1038/s41385-018-0061-0

The NLR family pyrin domain containing 11 protein contributes to the regulation of inflammatory signalling.

Ellwanger K, Becker E, Kienes I, Sowa A, Postma Y, Gloria YC, Weber ANR, Kufer TA.

J Biol Chem. 2018 Feb 23;293(8):2701-2710. doi: 10.1074/jbc.RA117.000152

NOD-like Receptors

Arnold, Christine; Kienes, Ioannis; Sowa, Anna S; and Kufer, Thomas A

eLS. John Wiley & Sons, Ltd: Chichester. doi: 10.1002/9780470015902.a0026236

2017

Impact of breakfast skipping compared with dinner skipping on regulation of energy balance and metabolic risk.

Nas A, Mirza N, Hägele F, Kahlhöfer J, Keller J, Rising R, Kufer TA, Bosy-Westphal A.

Am J Clin Nutr. 2017 May 10, doi: 10.3945/ajcn.116.151332

NOD1 modulates IL-10 signalling in human dendritic cells.

Neuper T, Ellwanger K, Schwarz H, Kufer TA, Duschl A, Horejs-Hoeck J.

Sci Rep. 2017 Apr 21;7(1):1005, doi: 10.1038/s41598-017-00691-x

NLRC5 Functions beyond MHC I Regulation-What Do We Know So Far?

Benkö S, Kovács EG, Hezel F, Kufer TA

Front Immunol. 2017 Feb 17;8:150., doi: 10.3389/fimmu.2017.00150

A high-sensitivity, bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real-time.

Kuri P, Ellwanger K, Kufer TA, Leptin M, Bajoghli B

J Cell Sci. 2017, Feb 1; 130(3), doi: 10.1242/jcs.196485

2016

Epidermal NLRP10 contributes to contact hypersensitivity responses in mice.

Damm A, Giebeler N, Zamek J, Zigrino P, Kufer TA.

EUR J Immunol. 2016 May 25. doi:10.1002/eji.201646401. [Epub ahead of print]

NOD2 dependent neutrophil recruitment is required for early protective immune responses against infectious Litomosoides sigmodontis L3 larvae

Ajendra J, Specht S, Ziewer S, Schiefer A, Pfarr K, Parčina M, Kufer TA, Hoerauf A, Hübner MP.

Sci Rep. 2016 Dec 22 doi: 10.1038/srep39648

NLRC5 elicits antitumor immunity by enhancing processing and presentation of tumor antigens to CD8+ T lymphocytes

Galaxia M. Rodriguez, Diwakar Bobbala, Daniel Serrano, Marian Mayhue, Audrey Champagne, Caroline Saucier, Viktor Steimle, Thomas A. Kufer, Alfredo Menendez, Sheela Ramanathan & Subburaj Ilangumaran

Oncoimmunology. 2016 Mar 28;5(6):e1151593. doi: 10.1080/2162402X.2016.1151593

Multifaceted Functions of NOD-Like Receptor Proteins in Myeloid Cells at the Intersection of Innate and Adaptive Immunity

Thomas A. Kufer, Guilia Nigro, Philippe J. Sansonetti

Microbiol. Spetr. Aug 4(4), doi: 10.1128/microbiolspec.MCHD-0021-2015

2015

http://zellbiologie.de/cellnews/archive/CellNews_01_15/index.html#1

Sensing of pathogen-induced F-actin perturbations – a new paradigm in innate immunity?

Angelika Hausser, Kornelia Ellwanger & Thomas Kufer;

Cell News 1/2015 (Deutsche Gesellschaft für Zellbiologie)

http://www.ncbi.nlm.nih.gov/pubmed/25404059

NLRC5 interacts with RIG-I to induce a robust antiviral response against influenza virus infection.

Ranjan P, Singh N, Kumar A, Neerincx A, Kremmer E, Cao W, Davis WG, Katz JM, Gangappa S, Lin R, Kufer TA, Sambhara S.;

EUR J Immunol. 2015 Mar; 45(3):758-72.

PRR-signaling pathways – Learning from microbial tactics

Sellge G, Kufer TA;

Seminars in Immunology 2015 Apr 22. pii: S1044-5323(15)00017-2. doi: 10.1016/j.smim.2015.03.009.

http://www.ncbi.nlm.nih.gov/pubmed/26124759

NLR-protein functions in immunity

Fritz JH, Kufer TA;

Front Immunol. 2015 Jun 11; 6 306

2014

http://www.ncbi.nlm.nih.gov/pubmed/25187968

The Cofilin Phosphatase Slingshot Homolog 1 (SSH1) Links NOD1 Signaling to Actin Remodeling.

Bielig H, Lautz K, Braun PR, Menning M, Machuy N, Brügmann C, Barisic S, Eisler SA, Andree M, Zurek B, Kashkar H, Sansonetti PJ, Hausser A, Meyer TF, Kufer TA.

PLoS Pathog. 2014 Sep 4;10(9):e1004351. doi: 10.1371/journal.ppat.1004351. eCollection 2014 Sep.

The N-Terminal Domain of NLRC5 Confers Transcriptional Activity for MHC Class I and II Gene Expression.

Neerincx A, Jakobshagen K, Utermöhlen O, Büning H, Steimle V, Kufer TA.

J Immunol. 2014 Sep 15;193(6):3090-100. doi: 10.4049/jimmunol.1401065.

http://www.ncbi.nlm.nih.gov/pubmed/25056906

BID-dependent release of mitochondrial SMAC dampens XIAP-mediated immunity against Shigella.

Andree M, Seeger JM, Schüll S, Coutelle O, Wagner-Stippich D, Wiegmann K, Wunderlich CM, Brinkmann K, Broxtermann P, Witt A, Fritsch M, Martinelli P, Bielig H, Lamkemeyer T, Rugarli EI, Kaufmann T, Sterner-Kock A, Wunderlich FT, Villunger A, Martins LM, Krönke M, Kufer TA, Utermöhlen O, Kashkar H.

EMBO J. 2014 Jul 23. pii: e201387244. [Epub ahead of print]

Aggregatibacter actinomycetemcomitans Outer Membrane Vesicles Are Internalized in Human Host Cells and Trigger NOD1- and NOD2-Dependent NF-κB Activation.

Thay B, Damm A, Kufer TA, Wai SN, Oscarsson J.

Infect Immun. 2014 Oct;82(10):4034-46. doi: 10.1128/IAI.01980-14.

http://www.ncbi.nlm.nih.gov/pubmed/24958724

Engagement of nucleotide-binding oligomerization domain-containing protein 1 (NOD1) by receptor-interacting protein 2 (RIP2) is insufficient for signal transduction.

Mayle S, Boyle JP, Sekine E, Zurek B, Kufer TA, Monie TP.

J Biol Chem. 2014 Aug 15;289(33):22900-14. doi: 10.1074/jbc.M114.557900.

The immune receptor NOD1 and kinase RIP2 interact with bacterial peptidoglycan on early endosomes to promote autophagy and inflammatory signaling.

Irving AT, Mimuro H, Kufer TA, Lo C, Wheeler R, Turner LJ, Thomas BJ, Malosse C, Gantier MP, Casillas LN, Votta BJ, Bertin J, Boneca IG, Sasakawa C, Philpott DJ, Ferrero RL, Kaparakis-Liaskos M.

Cell Host Microbe. 2014 May 14;15(5):623-35. doi: 10.1016/j.chom.2014.04.001